autologous transplantation of adult mice spermatogonial stem cells into gamma irradiated testes

Autologous Transplantation of Adult Mice Spermatogonial Stem Cells into Gamma Irradiated Testes

OBJECTIVE We evaluated structural and functional changes of fresh and frozen-thawed adult mouse spermatogonial stem cells following auto-transplantation into gamma-irradiated testes. MATERIALS AND METHODS In this experimental research, the right testes from adult mice (n=25) were collected, then Sertoli and spermatogonial cells were isolated using two-step enzymatic digestion, lectin immobili...

Melatonin improves spermatogonial stem cells transplantation efficiency in azoospermic mice

Objective(s):Transplantation quality improvement and reduction of cellular damage are important goals that are now considered by researchers. Melatonin is secreted from the pineal gland and some organs such as testes. According to beneficial effects of melatonin (such as its antioxidant and antiapoptotic properties), researchers have proposed that the use of melatonin may improve transplantatio...

melatonin improves spermatogonial stem cells transplantation efficiency in azoospermic mice

objective(s):transplantation quality improvement and reduction of cellular damage are important goals that are now considered by researchers. melatonin is secreted from the pineal gland and some organs such as testes. according to beneficial effects of melatonin (such as its antioxidant and antiapoptotic properties), researchers have proposed that the use of melatonin may improve transplantatio...

Comparison of Neonatal and Adult Mice-derived Sertoli Cells in Support of Expansion of Mouse Spermatogonial Stem Cells In vitro

Melatonin improves spermatogonial stem cells transplantation efficiency in azoospermic mice

OBJECTIVE(S) Transplantation quality improvement and reduction of cellular damage are important goals that are now considered by researchers. Melatonin is secreted from the pineal gland and some organs such as testes. According to beneficial effects of melatonin (such as its antioxidant and antiapoptotic properties), researchers have proposed that the use of melatonin may improve transplantatio...

Phenotypic and molecular characterization of spermatogonial stem cells in adult primate testes.

BACKGROUND Knowledge about the identity and characteristics of spermatogonial stem cells (SSCs) in human is very limited. Here, Rhesus monkey was used as an animal model to investigate molecular and phenotypic characteristics of SSCs in the adult testes. METHODS A variety of immunohistological, molecular biological and functional assays were used to study different populations of SSCs in the ...